A sensitive and practical immunoradiometric assay of thyrotropin.
نویسندگان
چکیده
منابع مشابه
Thyrotropin as measured by a sensitive immunoradiometric assay.
An immunoradiometric assay (Boots-Celltech's "Sucrosep"; IRMA) for thyrotropin (TSH) was evaluated and results were compared with those of our in-house RIA procedure. The IRMA had a sensitivity of 0.02 milli-int. unit/L. In addition it displayed excellent intra- and inter-batch precision, cross-reacted negligibly with other pituitary hormones, and appeared to be relatively free of matrix effect...
متن کاملDevelopment and utility of a monoclonal-antibody-based, highly sensitive immunoradiometric assay of thyrotropin.
Using improved selection techniques, we isolated four monoclonal antibodies with high affinity for human thyrotropin (Ka = 1.6 X 10(8) to 2.6 X 10(10) L/mol). We used two of these in an immunoradiometric assay (IRMA) that also incorporates a novel phase-separation technology (Sucrosep TSH IRMA, Boots-Celltech). This assay's very low detection limit for TSH (0.03-0.08 milli-int. unit/L) and wide...
متن کاملAn automated immunoradiometric assay for human thyrotropin.
In this two-site immunoradiometric assay for thyrotropin, developed for use in the "Kemtek 3000" automated radioimmunoassay system, commercially available monoclonal antibody to thyrotropin is labeled with 125I, and the solid-phase antibody is an IgG fraction of sheep antiserum to thyrotropin, covalently coupled to reprecipitated aminocellulose. There are two incubations, totalling 3 h, the sen...
متن کاملDiagnostic value of thyrotropin concentrations in serum as measured by a sensitive immunoradiometric assay.
A new, highly sensitive immunoradiometric thyrotropin (TSH) assay involving solid-phase-coupled monoclonal antibodies (Boots-Celltech Sucrosep IRMA-TSH) has been evaluated in a wide variety of patients with thyroidal and nonthyroidal illnesses and the results compared with those obtained by conventional diagnostic TSH RIAs. The sensitivity of the present assay ranged from 0.036 to 0.1 milli-int...
متن کاملSensitive human thyrotropin immunoradiometric assay set up by the identification and minimization of nonspecific bindings.
An immunoradiometric assay (IRMA) of human thyrotropin (hTSH), based on magnetic solid phase separation, was studied especially in terms of its nonspecific bindings (B0) which were identified as a product of the interaction between an altered form of radioiodinated anti-hTSH monoclonal antibody (125I-mAB) and the uncoupled magnetizable cellulose particle (matrix). Preincubation with the same ma...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1986
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/32.3.514